A net anime about the Nijisanji VTuber duo ChroNoiR is in development. It will come out in 2023. The title will be ChroNoiR Episode 0 and you can see the official website here. ChroNoiR consists of Kanae and Kuzuha, who are two fairly famous and popular streamers from Nijisanji.
ChroNoiR Episode 0 will come out on the same day as their new song. It will depict their “first meeting,” which happened 150 years ago. Kanae and Kuzuha will voice themselves. According to the summary, it takes place in a town that has been dealing with a disease that turns people into the undead. However, ever since Kanae (who is a priest) moved nearby, cases have been on the decline. One day, a mysterious vampire (Kuzuha) starts appearing around Kanae.
Mikkun is the character designer for ChroNoiR Episode 0. They have previously designed a new outfit for Kanae’s VTuber model, as well as illustrated art of other Nijisanji livers. Mikkun has also released a key visual for the ChroNoiR ONA (original net animation):
In terms of the actual VTuber duo, Kanae and Kuzuha formed ChroNoiR in July 2018. It has its own YouTube channel. The two of them mostly upload songs that they sang together, and they have often streamed together as well. You can also watch stream clips featuring the duo from the ChroNoiR channel.
The Nijisanji ChroNoiR Episode 0 Vtuber anime is in development and will air some time in 2023.